Unraveling the Metabolite Signature of Endophytic Bacillus velezensis Strain Showing Defense Response towards Fusarium oxysporum

Seedling blight, caused by the fungus Fusarium oxysporum, significantly lowers rice production globally. Earlier reports have opined that endophytic bacteria strains could be possible biocontrol agents, but mechanistic actions involved are still unclear. Therefore, this study aimed to isolate with high inhibitory activity and elucidate its mechanisms for inducing resistance metabolomics. The results showed mdj-36 had strongest in vitro pathogen inhibition of F. while mdj-34 displayed lowest identified as Bacillus velezensis strains. Metabolomic analyses demonstrated B. growth medium produce higher organic acids, terpenes, diterpene than mdj-34. Further investigation revealed ‘secondary bile acid biosynthesis’ ‘glycerophospholipid metabolism’ pathways played essential roles defense response towards oxysporum. This study’s findings provide a credible theoretical basis use strain against seedling blight.